Comparison of point detection and area detection for point-scanning structured illumination microscopy

Comparison of point detection and area detection for point-scanning structured illumination microscopy

Blog Article

Structured illumination microscopy (SIM) is suitable for Pre-Rolls biological samples because of its relatively low-peak illumination intensity requirement and high imaging speed.The system resolution is affected by two typical detection modes: Point detection and area detection.However, a systematic analysis of the imaging performance of the different detection modes of the system has rarely been conducted.In this study, we compared laser point scanning point detection (PS-PD) and point scanning area detection (PS-AD) imaging in Supplies nonconfocal microscopy through theoretical analysis and simulated imaging.The results revealed that the imaging resolutions of PS-PD and PS-AD depend on excitation and emission point spread functions (PSFs), respectively.

Especially, we combined the second harmonic generation (SHG) of point detection (P-SHG) and area detection (A-SHG) with SIM to realize a nonlinear SIM-imaging technique that improves the imaging resolution.Moreover, we analytically and experimentally compared the nonlinear SIM performance of P-SHG with that of A-SHG.